Identification of unique bacterial gene segments from Streptococcus mutans with potential relevance to dental caries by subtraction DNA hybridization.
نویسندگان
چکیده
Using DNA subtractive hybridization, 49 unique gene segments were identified from a strain of Streptococcus mutans that was isolated from a patient with severe early childhood caries (S-ECC). Further hybridization with DNA from other S. mutans strains isolated from both caries-active and caries-free subjects yielded five unique sequences of DNA common to strains associated with S-ECC.
منابع مشابه
Improvement in curcumin application for preventing dental caries by embeding in nanoparticles
Introdution: The aim of this research was optimization curcumin characteristics for oral hygiene application. Curcumin-loaded starch nano-particles were developed for enhancing adhesion propery with enamel surface and best anti-bacterial effect against Streptococcus mutans. Methods: The study was the experimental one. The nanoparticles synthesize was based on precipitation and ionic gelation m...
متن کاملThe Frequency of Streptococcus Mutans and Lactobacillus spp.in 3-5-year- old Children with and without Dental Caries
Abstract Background and Objective: The high occurrence of early childhood caries (ECC) is one of the most common problems in children dentistry. Streptococcus mutans and lactobacilli.spp are closely associated with the development of early childhood caries (ECC). Therefore, the aim of this study was to investigate the frequency of streptococcus mutans and lactobacilli.spp in 3-5 –year- old ch...
متن کاملMicrobial risk indicators of early childhood caries.
The aim of this study was to use molecular identification methods, such as 16S RNA gene sequence and reverse-capture checkerboard hybridization, for identification of the bacteria associated with dental caries and with dental health in a subset of 204 twins aged 1.5 to 7 years old. A total of 448 plaque samples (118 collected from caries-free subjects and 330 from caries-active subjects) were u...
متن کاملEffects of Short-term Consumption of Probiotic Yogurt on Streptococcus Mutans and lactobacilli Levels in 18-30 Years Old Students with Initial Stages of Dental Caries in Ahvaz City
Background and Objectives: Dental caries, caused by oral microbial flora, is considered as one of the most common infectious diseases in human. The aim of this study was to determine the effect of short-term consumption of probiotic yogurt containing bifidobacterium lactis on salivary streptococcus mutans and lactobacilli in students with initial stages of dental caries. Materials and Methods:...
متن کاملIdentification and Detection of Streptococcus MUTANs in Plaque Samples
Streptococcus mutans have been proposed as the main causative agent of dental cavities. Dental caries is among the most common diseases in the world, this study was done to determine whether PCR analysis is useful to identify and even classify oral and other streptococci. 20 dental plaque samples were collected from teeth, 10 normal sample and 10 from persons with oral problems. The samples wer...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 43 7 شماره
صفحات -
تاریخ انتشار 2005